S-STEM Scholar Evan Russell's Blog

  Introduction     The attractants are not working. We shall move on to new attractants later, but in the interest of time we are shifting the focus of the poster to giving a general characterization of twitch within Deinococcus. We shall explore twitch from: P81, Ficus, Xin, Pimensis, radiodurans, gobiensis and indicus. Methods Twitch Motility Assay Protocol 1) Select media (e.g. 0.75% SMA and 0.5% agar) 2) Prepare a sterile hood by wiping everything down with ethanol 3) Melt media down 4) Wipe down all inoculating materials and insert them into the sterile hood 5) Dispense 10 ml of media into empty petri dishes containing five pronged molds to allow for insertion sites a. Use a sterile 10 ml pipette 6) Let plates cool 7) Remove molds 8) Use UV light for 15 minutes to ensure sterilization of plates  9) Inoculate center of plate (directly onto the plastic) 10) Dispense 100-150 microliters of chemoattractant media into one of the insertion sites ...

Introduction     The attempt of standardizing the initial inoculate has led to too many new issues and thus must be discarded. The initial pellet keeps falling apart within the eppendorf tube. We shall go back to drawing large colonies from plates.  Methods Twitch Motility Assay Protocol 1) Select media (e.g. 0.75% SMA and 0.5% agar) 2) Prepare a sterile hood by wiping everything down with ethanol 3) Melt media down 4) Wipe down all inoculating materials and insert them into the sterile hood 5) Dispense 10 ml of media into empty petri dishes containing five pronged molds to allow for insertion sites a. Use a sterile 10 ml pipette 6) Let plates cool 7) Remove molds 8) Use UV light for 15 minutes to ensure sterilization of plates  9) Inoculate center of plate (directly onto the plastic) 10) Dispense 100-150 microliters of chemoattractant media into one of the insertion sites a. Use 1% agar media 11) Incubate Plates 12) Regularly Picture Resa...